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Curriculum vitae di CALLIGARI

Paolo Calligari (nato a Novara il 20 agosto 1964) diploma di Perito Agrario conseguito nel 1985 presso l'Istituto Tecnico Agrario Statale "G.Bonfantini"di Novara. Nel 1988 è assunto dalla Società Agricola e Forestale (SAF) del gruppo Ente Nazionale per la Cellulosa e la Carta presso la sezione di Miglioramento Genetico dell'Istituto di Sperimentazione per la Pioppicoltura.

Attività
Dal 1988 al 1997: Tecnico di ricerca della sezione di Miglioramento Genetico (realizzazione di impianti con schemi sperimentali, rilevamento dati dendrometrici in piantagioni e vivai, elaborazione dati tramite PC, realizzazione di incroci controllati in serra).
1998: Tecnico di ricerca assegnato al laboratorio di Biotecnologie.
Da Luglio 1999 a Luglio 2001: Gestione autonoma attività del laboratorio di Biotecnologie per vacanza ricercatore responsabile.
A tutt’oggi: Tecnico di ricerca assegnato al laboratorio di Biotecnologie.
Occupazioni principali: Micropropagazione e conservazione di colture ‘in vitro’, gestione e realizzazione prove di trasformazione su pioppo mediate da Agrobacterium tumefaciens ed applicazioni di biologia molecolare.
 
• Applicazione in laboratorio di diverse tecniche di biologia molecolare:
     PCR (Polymerase Chain Reaction)
     Estrazione di DNA plasmidico
     Estrazione di DNA genomico da tessuti vegetali
     Estrazione di RNA da tessuti vegetali
     Analisi elettroforetica su gel di agarosio
     Analisi elettroforetica di RNA su gel denaturante agarosio
• Allestimento ‘ex-novo’di protocolli idonei alla rigenerazione/trasformazione di varie specie di pioppo e salice (P.× canadensis, P.nigra, P.deltoides, P.trichocarpa, Salix ssp.)
• Coltura ‘in vitro’ di cellule e tessuti vegetali di cloni di pioppo e salice.
• Coltura ‘in vitro’ di cellule e tessuti vegetali di cloni commerciali di eucalitto.



Elenco pubblicazioni
BONADEI, M,; ZELASCO, S.; GIORCELLI, A.; GENNARO, M.; CALLIGARI, P.; QUATTRINI, E.; CARBONERA, D.; BALESTRAZZI, A. 2012 Transgene stability and agronomical performance of two transgenic Basta-tolerant lines pf Populus alba.
Plant Biosystems 146: 33-40 [En]
BALESTRAZZI, A.; BONADEI, M. ZELASCO, S.; GIORCELLI, A.; GENNARO, M.; CALLIGARI, P.; MATTIVI, F.; QUATTRINI, E.; CARBONERA, D. 2011 Seasonal and tissue-specific transgene exprtession and resveratrol-3-glucoside (piceid) accumulation in genetically modified white poplars carrying the grapewine StSy gene.
Plant Cell Tissue Organ Culture 1-8 [En]
NERVO G.; CALLIGARI P.; COLLOT T.; ZELASCO S.; CARRA A. 2011 Genetic transformation of poplar clones for useful traits, in compliance to the biosafety law.
Actas del Tercer Congreso Internacional de las Salicáceas en Argentina 'Los álamos y los sauces junto al paisaje y el desarrollo productivo de la Patagonia' Neuquen, Argentina 16-19 Marzo 2011. 1 p. [En]
BALESTRAZZI, A.; BOTTI, S.; ZELASCO, S.; BIONDI, S.; FRANCHIN, C.; CALLIGARI, P.; RACCHI, M.; TURCHI, A.; LINGUA, G.; BERTA, G.; CARBONERA, D. 2009 Expression of the PsMTa1 gene in white poplar engineered with the MAT system is associated with heavy metal tolerance and protection against 8-hydroxy-2'-deoxyguanosine mediated -DNA damage.
Plant Cell Report [En]
CALLIGARI, P.; COLLOT, T.; ZELASCO, S.; NERVO, G. 2008 Genetic transformation of elite poplar clones for useful traits. In: IPC 23rd Sess. 'Poplars, Willows and People's Wellbeing'. Abstracts of Submitted Papers. Beijing, China, 27-30 October 2008. p. 28 [En]
CALLIGARI, P.; COLLOT, T.; ZELASCO, S.; NERVO, G. 2008 Genetic transformation of elite poplar clones for useful traits.
Presented at the IPC 23rd Sess. 'Poplars, Willows and People's Wellbeing'. Abstracts of Submitted Papers. Beijing, China, 27-30 October 2008. [En]
NERVO, G.; CALLIGARI, P.; ACQUADRO, A.; PORTIS, E.; LANTERI, S. 2008 Application of SSR markers for DNA fingerprinting of commercial poplar clones. In: IPC 23rd Sess. 'Poplars, Willows and People's Wellbeing'. Abstracts of Submitted Papers. Beijing, China, 27-30 October 2008. p. 138 [En]
NERVO, G.; CALLIGARI, P.; ACQUADRO, A.; PORTIS, E.; LANTERI, S. 2008 Application of SSR markers for DNA fingerprinting of commercial poplar clones.
IPC 23rd Sess. 'Poplars, Willows and People's Wellbeing'. Abstracts of Submitted Papers. Beijing, China, 27-30 October 2008. 30 pp. [En]
ZELASCO, S.; GIORCELLI, A.; BALESTRAZZI,A.; MATTIVI, F.; CONFALONIERI, M.; CARBONERA, D.; BONADEI, M.; QUATTRINI, E.; PICCO, F.; CALLIGARI, P.; DEANDREA, G.; GENNARO, M. 2007 Evaluation of agronomic performance, molecular and transgene-soil interactions of GM poplars. In: Proceedings of the 51st Italian Society of Agricultural Genetics Annual Congress. Riva del Garda, Italy 23/26 September 2007. G. 24 [En]
ZELASCO, S.; GIORCELLI, A.; BALESTRAZZI,A.; MATTIVI, F.; CONFALONIERI, M.; CARBONERA, D.; BONADEI, M.; QUATTRINI, E.; PICCO, F.; CALLIGARI, P. 2007 Evaluation of agronomic performance, molecular and transgene-soil interactions of GM poplars.
Presented at the 51st Italian Society of Agricultural Genetics Annual Congress. Riva del Garda, Italy 23/26 September 2007. [En]
ZELASCO, S.; PIETRA, S.; FORNASIER, F.; COLLOT, T.; CALLIGARI, P.; NERVO G.; CONFALONIERI, M. 2007 Effect of cadmium on the elite white poplar clone 'Villafranca'. In: Proceedings of the 51st Italian Societty of Agricultural Genetics Annual Congress. Riva del Garda, Italy 23/26 September 2007. C. 10 [En]
ZELASCO, S.; PIETRA, S.; FORNASIER, F.; COLLOT, T.; CALLIGARI, P.; NERVO G.; CONFALONIERI, M. 2007 Effect of cadmium on the root growth of the elite white poplar clone 'Villafranca'.
Presented at the 51st Italian Societty of Agricultural Genetics Annual Congress. Riva del Garda, Italy 23/26 September 2007. [En]
ZELASCO, S.; RESSEGOTTI, V.; CONFALONIERI, M.; CARBONERA, D.; CALLIGARI, P.; BONADEI, M.; BISOFFI, S.; YAMANDA, K.; BALESTRAZZI, A. 2007 Evaluation of MAT-vector system in white poplar (Populus alba L.) and production of ipt marker-free transgenic plants by 'single-step transformation'.
Plant Cell Tissue and Organ Culture 61-72 [En]
CALLIGARI, P.; DELIA, G.; ZELASCO, S.; CONFALONIERI, M.; BALESTRAZZI, A.; YAMADA, K.; COLLOT, T.; NERVO, G. 2006 In vitro regeneration and genetic transformation of P. xcanadensis clones.
Presented at the 11th IAPTC&B International Congress of Plant Tissue culture and Biotechnology. Beijing, China 13-18 August 2006.
CALLIGARI, P.; DELIA, G.; ZELASCO, S.; CONFALONIERI, M.; BALESTRAZZI, A.; YAMADA, K.; COLLOT, T.; NERVO, G. 2006 In vitro regeneration and genetic transformation of P. xcanadensis clones. In: 11th IAPTC&B Congress. Biotechnology and Sustainable Agriculture 2006 and Beyond. 2006. Beijing, China, August 13-18. 69 [En]
CARBONERA, D.; BALESTRAZZI, A.; BOTTI, S.; ZELASCO, S.; BIONDI, S.; LINGUA, G.; FRANCHIN, C.; CESARO, P.; CALLIGARI, P. 2006 Expression of the pea metallothionein 2A gene in white poplar: implication for heavy metal stress response.
Presented at the 'XV FESPB Congress of European Societies of Plant Biology' Lyon, 17-21 july 2006. [En]
CARBONERA, D.; BALESTRAZZI, A.; BOTTI, S.; ZELASCO, S.; BIONDI, S.; LINGUA, G.; FRANCHIN, C.; CESARO, P.; CALLIGARI, P. 2006 Expression of the pea metallothionein 2A gene in white poplar: implication for heavy metal stress response. In: XV FESPB Congress Federation of European Societies of Plant Biology: Book of Abstracts, Lyon , France, 17-21 july 2006. p. 162 [En]
DELIA, G.; NERVO, G.; ZELASCO, S.; BALESTRAZZI, A.; PIETRA, S.; COLLOT, T.; YAMANDA, K.; CALLIGARI, P. 2006 Genetic transformation of elite P.x canadensis clones with a morphological marker gene. In: Proceedings of the 50th Italian Society of Agricultural Genetics Annual Congress. Ischia, Italy 10-14 September 2006. E.10 [En]
DELIA, G.; NERVO, G.; ZELASCO, S.; BALESTRAZZI, A.; PIETRA, S.; COLLOT, T.; YAMANDA, K.; CALLIGARI, P. 2006 Genetic transformation of elite P.x canadensis clones with a morphological marker gene.
Presented at the 50th Italian Society of Agricultural Genetics Annual Congress. Ischia, Italy 10-14 September 2006. [En]
ZELASCO, S.; BALESTRAZZI, A.; MATTIVI, F.; BONADEI, M.; CONFALONIERI, M.; CARBONERA, D.; QUATTRINI, E.; CALLIGARI, P.; DEANDREA, G.; PICCO, F.; GIORCELLI, A. 2006 GM poplar cultivation: evaluation of agronomic performance, molecular and biochemical investigations, transgene-soil interactions. In: Proceedings of the 50th Italian Society of Agricultural Genetics Annual Congress, Ischia, 10/14 settembre 2006. Poster abstract.. G.21 [En]
ZELASCO, S.; BALESTRAZZI, A.; MATTIVI, F.; BONADEI, M.; CONFALONIERI, M.; CARBONERA, D.; QUATTRINI, E.; CALLIGARI, P.; DEANDREA, G.; PICCO, F.; GIORCELLI, A. 2006 GM poplar cultivation: evaluation of agronomic performance, molecular and biochemical investigations, transgene-soil interactions.
Presented at the 50th Italian Society of Agricultural Genetics Annual Congress. Ischia, Italy - 10-4 September 2006. [EN]
ZELASCO, S.; CONFALONIERI, M.; BALESTRAZZI, A.; MATTIVI, F.; CARBONERA, D.; QUATTRINI, E.; BONADEI, M.; CALLIGARI, P.; DEANDREA, G.; PICCO, F.; GIORCELLI, A. 2006 Evaluation of GM poplars expressing relevant traits for herbicide tolerance, disease resistance and production of pharmaceutics: biochemical, molecular and microbiological studies on plants and detection of transgene sequences in soil. In: Biotechnology and Sustainable Agriculture 2006 and Beyond. Proceedings of the 11th IAPTC&B Congress. Beijing, China, 13-18 August 2006. 413-418 [En]
ZELASCO, S.; CONFALONIERI, M.; BALESTRAZZI, A.; MATTIVI, F.; CARBONERA, D.; QUATTRINI, E.; BONADEI, M.; CALLIGARI, P.; DEANDREA, G.; PICCO, F.; GIORCELLI, A. 2006 Evaluation of GM poplars expressing relevant traits for herbicide tolerance, disease resistance and production of pharmaceutics: biochemical, molecular and microbiological studies on plants and detection of transgene sequences in soil. In: 11th IAPTC&B Congress. Biotechnology and Sustainable Agriculture 2006 and Beyond. Abstracts Beijing, China, August 13-18, 2006. p. 21 [En]
ZELASCO, S.; REGGI, S.; CALLIGARI, P.; BALESTRAZZI, A.; BONGIORNI, C.; QUATTRINI, E.; DELIA, G.; BISOFFI, S.; FOGHER, C.; CONFALONIERI, M. 2006 Expression of the Vitreoscilla hemoglobin (Vhb)-encoding gene in transgenic white poplar: plant growth and biomass production, biochemical characterization and cell survival under submergence, oxidative and nitrosative stress conditions.
Molecular Breeding 201-216 [En]
CALLIGARI, P.; DELIA, G., ZELASCO, S.; CONFALONIERI, M.; BOTTI, S.; PIETRA, S.; COLLOT, T.; NERVO, G: 2005 New methods for in vitro regeneration and genetic modification of elite populus euramericana clones. In: SIGA - Società Italiana Genetica Agraria - XLIX ANNUAL CONGRESS, Scientific Program and Poster List. Università di Potenza,12-15 Settembre 2005. [En]
CALLIGARI, P.; DELIA, G., ZELASCO, S.; CONFALONIERI, M.; BOTTI, S.; PIETRA, S.; COLLOT, T.; NERVO, G: 2005 New methods for in vitro regeneration and genetic modification of elite Populus euramericana clones.
Presented at the SIGA - Società Italiana Genetica Agraria - XLIX ANNUAL CONGRESS, Scientific Program, Poster List. Università di Potenza, 12-15 Settembre 2005. [En]
CONFALONIERI, M.; BALESTRAZZI, A.; MATTIVI, F. CARBONERA, D.; ZELASCO S.; QUATTRINI, E.; BONADEI, M.; CALLIGARI, P.; DEANDREA, G.; PICCO, F.; GIORCELLI, A.; GENNARO, M. 2005 Evaluation of GM poplar expressing relevant traits for herbicide tolerance, disease resistance and production of pharmaceutics: biochemical, molecular and microbiological studies on plants and detection of transgene sequences in soil. In: SIGA - Società Italiana Genetica Agraria - XLIX ANNUAL CONGRESS, Scientific Program, Poster List. Università di Potenza, 12-15 Settembre 2005.
CONFALONIERI, M.; BALESTRAZZI, A.; MATTIVI, F. CARBONERA, D.; ZELASCO S.; QUATTRINI, E.; BONADEI, M.; CALLIGARI, P.; DEANDREA, G.; PICCO, F.; GIORCELLI, A. 2005 Evaluation of GM poplar expressing relevant traits for herbicide tolerance, disease resistance and production of pharmaceutics: biochemical, molecular and microbiological studies on plants and detection of transgene sequences in soil.
Presented at the SIGA - Società Italiana Genetica Agraria - XLIX ANNUAL CONGRESS, Scientific Program, Poster List. Università di Potenza, 12-15 Settembre 2005.
BONADEI, M.; BALESTRAZZI, A.; CONFALONIERI, M.; ZELASCO, S.; CALLIGARI, P.; RESSEGOTTI, V.; BISOFFI, S.; CARBONERA, D. 2004 GM plants and environment: a study to assess the occurrence of horizontal gene transfer between transgenic forest trees (Populus alba L.) and soil microrganisms. In: Riunione annuale gruppi Biotecnologie-Differenziamento e Biologia Cellulare-Molecolare. Belgirate (VB) 23-25 Giugno 2004. p. 26 [En]
GIORCELLI, A.; SPARVOLI, F.; MATTIVI, F.; TAVA, A.; BALESTRAZZI, A.; VRHOVSEK, U.; CALLIGARI, P.; BOLLINI, R.; CONFALONIERI, M. 2004 Expression of the stilbene synthase (StSy) gene from grapevine in transgenic white poplar results in high accumulation of the antioxidant resveratrol glucosides.
Transgenic Research 13: 203-214 [En]
RESSEGOTTI, V.; ZELASCO, S.; BONADEI, M.; BALESTRAZZI, A.; CONFALONIERI, M.; CARBONERA, D.; CALLIGARI, P.; YAMADA, K.; BISOFFI, S. 2004 Detection of transgene sequences in soils cultivated with GM poplars and production of 'marker-free' GM poplar plants: different strategies to evaluate and improve the environmental impact of transgenic trees.
Acta physiologiae plantarum, Special Issue: The 14th FESPB Congress Book of Abstracts. August 23-27, 2004 Cracow, Poland 26: Supplement, [En]
RESSEGOTTI, V.; ZELASCO, S.; BONADEI, M.; BALESTRAZZI, A.; CONFALONIERI, M.; CARBONERA, D.; CALLIGARI, P.; YAMADA, K.; BISOFFI, S. 2004 Detection of transgene sequences in soils cultivated with GM poplars and production of 'marker-free' GM poplar plants: different strategies to evaluate and improve the environmental impact of transgenic trees.
Presented at the 14th FESPB Congress. August 23-27, 2004 Cracow, Poland. [En]
ZELASCO, S.; FOGHER, C.; CALLIGARI, P.; SAVAZZINI, F.; BISOFFI, S.; PIETRA, S.; BOLCHI, A.; SCARAMELLI, L.; OTTONELLO, M. CONFALONIERI, M. 2004 Expression of an artificial metallothionein gene in transgenic white poplar and evaluation of Cadmium tolerance. In: Proceedings of SIFV - SIGA Joint congress . SIFV XLIII Annual Congress - SIGA XLVIII Annual Congress. Lecce, 15-18 September 2004. p. 223 [En]
ZELASCO, S.; FOGHER, C.; CALLIGARI, P.; SAVAZZINI, F.; BISOFFI, S.; PIETRA, S.; BOLCHI, A.; SCARAMELLI, L.; OTTONELLO, M.; CONFALONIERI, M. 2004 Expression of an artificial phytochelatin gene in transgenic white poplar and evaluation of cadmium tolerance.
Presented at the SIFV - SIGA Joint congress . SIFV XLIII Annual Congress - SIGA XLVIII Annual Congress. Lecce, 15-18 September 2004. [En]
ZELASCO, S.; REGGI, S.; BISOFFI, S.; BONGIORNI, C.; CALLIGARI, P.; BALESTRAZZI, A.; FOGHER, C.; CONFALONIERI, M. 2003 Expression of the Vitreoscilla hemoglobin (Vhb)-encoding gene in transgenic white poplar: biochemiical characterization and effects on plant growth rate and biomass production. In: SIGA XLVII Annual Congress. Scientific Programme & Poster List. Verona 24-27 September 2003. p. 22 [En]
ZELASCO, S.; REGGI, S.; BISOFFI, S.; BONGIORNI, C.; CALLIGARI, P.; BALESTRAZZI, A.; FOGHER, C.; CONFALONIERI, M. 2003 Expression of the Vitreoscilla hemoglobin (Vhb)-encoding gene in transgenic white poplar: biochemiical characterization and effects on plant growth rate and biomass production.
Presented at the SIGA XLVII Annual Congress. Scientific Programme & Poster List. Verona 24-27 September 2003. p. 22 [En]
BALESTRAZZI, A.; CONFALONIERI, M.; RESSEGOTTI, V.; CALLIGARI, P.; ANFOSSO, L.; SKINNER, J.; STRAUSS, S.; CARBONERA, D. 2002 Genetic engineering of sexual sterility in poplar. In: Società Botanica Italiana, Riunione Congiunta gruppi di Lavoro Biotecnologie e Differenziamento Biologia Cellulare e Molecolare, Verona, 12-14 giugno 2002. p. 20 [En]
CARBONERA, D.; BALESTRAZZI, A.; CONFALONIERI, M.; RESSEGOTTI, V.; ANFOSSO. L.; CALLIGARI, P.; SKINNER, J.; STRAUSS, S. 2002 Control of reproduction in Populus alba by genetic ablation of floral tissues. In: International Poplar Symposium III on basic and applied aspects of poplar and willow biology. Uppsala, Sweden. August 26-29, 2002. p. 222 [En]
CONFALONIERI, M.; REGGI, S.; ANFOSSO, L.; .BALESTRAZZI, A.; CALLIGARI, P.; BONGIORNI, C.; FOGHER, C. 2002 Genetic transformation of White Poplar (Populus alba L.) with a Vitreoscilla Hemoglobin (Vhb)-encoding Gene and evaluation of growth rate and biomass production. In: X IAPTC&B Congress 'Plant Biotechnology 2002 and Beyond' A Celebration and a Showcase. Orlando, Florida. June 23-28, 2002. p. 65-A [EN]
CONFALONIERI, M.; REGGI, S.; ANFOSSO, L.; .BALESTRAZZI, A.; CALLIGARI, P.; BONGIORNI, C.; FOGHER, C. 2002 Genetic transformation of White Poplar (Populus alba L.) with a Vitreoscilla Hemoglobin (Vhb)-encoding Gene and evaluation of growth rate and biomass production.
Presented at the X IAPTC&B Congress 'Plant Biotechnology 2002 and Beyond' A Celebration and a Showcase. Orlando, Florida. June 23-28, 2002. [EN]
DELLEDONNE, M.; ALLEGRO, G.; BELENGHI, B.; BALESTRAZZI, A.; PICCO, F.; LEVINE, A.; ZELASCO, S.; CALLIGARI, P.; CONFALONIERI, M. 2001 Transformation of white poplar (Populus alba L.) with a novel Arabidopsis thaliana cysteine proteinase inhibitor and analysis of insect pest resistance.
Molecular Breeding 7: 35-42 [En]
CONFALONIERI, M.; SPARVOLI, F.; BALESTRAZZI, A.; CALLIGARI, P.; BOLLINI, R. 2000 Transformation of elite white poplar (Populus alba L.) with stilbene synthase-encoding gene using Agrobacterium tumefaciens. In: 21st Session of the International Poplar Commission (IPC 2000) Poplar and Willow culture: Meeting the Needs of Society and the Environment [Isebrands, J.G.; Richardson, J. comps.] Gen. Tech. Rep. p. 39 [En]
CONFALONIERI, M.; SPARVOLI, F.; BALESTRAZZI, A.; CALLIGARI, P.; BOLLINI, R. 2000 Transformation of elite white poplar (Populus alba L.) with stilbene synthase-encoding gene using Agrobacterium tumefaciens. In: 21st Session of the International Poplar Commission (IPC 2000) Poplar and Willow culture: Meeting the Needs of Society and the Environment [Isebrands, J.G.; Richardson, J. comps.] Gen. Tech. Rep. p. 39 [En]
DELLEDONNE, M.; ALLEGRO, G.; BELENGHI, B.; BALESTRAZZI, A.; PICCO, F.; ZELASCO, S.; CALLIGARI, P.; CONFALONIERI, M. 2000 Trasformazione genetica di Populus alba L con una cistatina di Arabidopsis thaliana ed analisi alla resistenza indotta. In: Riunione Congiunta gruppi di Lavoro Biologia Cellulare e Molecolare e Biotecnologie e Differenziamento della Società Botanica Italiana. Pavia, 14-16 giugno 2000. p. 23 [It]